Soft Ionization of Metallo-Mefenamic Using Electrospray Ionization Mass Spectrometry

Desorption electrospray ionization-mass spectrometry of proteins.

Desorption electrospray ionization-mass spectrometry (DESI-MS) was evaluated for the detection of proteins ranging in molecular mass from 12 to 66 kDa. Proteins were uniformly deposited on a solid surface without pretreatment and analyzed with a DESI source coupled to a quadrupole ion trap mass spectrometer. DESI-MS parameters optimized for protein detection included solvent flow rate, temperat...

Electrospray ionization mass spectrometry fingerprinting of beer.

After just simple degassing, dilution, pH adjustment and direct flow injection, characteristic fingerprint spectra of beer samples have been obtained by fast (few seconds) electrospray ionization mass spectrometry (ESI-MS) analysis in both the negative and positive ion modes. A total of 29 samples belonging to the two main beer types (lagers and ales) and several beer subtypes from USA, Europe ...

chromatography–electrospray ionization tandem mass spectrometry

Isoelectric Focusing, Matrix-assisted Laser Desorption/ionization Time-of- Flight Mass Spectrometry, Electrospray Ionization Mass Spectrometry

LEVEL: INTERMEDIATE D iscovery, development, and commercialization of novel biologics frequently involve collaboration between two or more companies. In the context of these business relationships, transfer of technology from one institution to another is a crucial step that needs to be executed f lawlessly and rapidly. Follow-up activities usually include the development of productive, reliabl...

Quantifying protein-fatty acid interactions using electrospray ionization mass spectrometry.

The application of the direct electrospray ionization mass spectrometry (ESI-MS) assay to quantify interactions between bovine β-lactoglobulin (Lg) and a series of fatty acids (FA), CH(3)(CH(2))(x)COOH, where x=6 (caprylic acid, CpA), 8 (capric acid, CA), 10 (lauric acid, LA), 12 (myristic acid, MA), 14 (palmitic acid, PA) and 16 (stearic acid, SA), is described. Control ESI-MS binding measurem...